pima cd4 cartridge  (Abbott Laboratories)

Journal: Biomedical Reports

Article Title: Identification of cytokine signatures in HIV‑infected individuals with and without Mycobacterium tuberculosis co‑infection

doi: 10.3892/br.2024.1819

Figure Lengend Snippet: Baseline characteristics of the study population.

Article Snippet: PIMA™ CD4 cartridge was inserted immediately into the Alere PIMA CD4 analyzer (Alere, Inc.) and CD4 + cell count was automatically calculated.

Techniques:

Journal: Biomedical Reports

Article Title: Identification of cytokine signatures in HIV‑infected individuals with and without Mycobacterium tuberculosis co‑infection

doi: 10.3892/br.2024.1819

Figure Lengend Snippet: Cytokine production stratified by CD4 count <350 cells/µl.

Article Snippet: PIMA™ CD4 cartridge was inserted immediately into the Alere PIMA CD4 analyzer (Alere, Inc.) and CD4 + cell count was automatically calculated.

Techniques:

Journal: Biomedical Reports

Article Title: Identification of cytokine signatures in HIV‑infected individuals with and without Mycobacterium tuberculosis co‑infection

doi: 10.3892/br.2024.1819

Figure Lengend Snippet: Cytokine production stratified by CD4 count >350 cells/µl.

Article Snippet: PIMA™ CD4 cartridge was inserted immediately into the Alere PIMA CD4 analyzer (Alere, Inc.) and CD4 + cell count was automatically calculated.

Techniques:

Journal: Biomedical Reports

Article Title: Identification of cytokine signatures in HIV‑infected individuals with and without Mycobacterium tuberculosis co‑infection

doi: 10.3892/br.2024.1819

Figure Lengend Snippet: IL-17A, IFN-γ, TNF, IL-10, IL-6, IL-4 and IL-2 levels in HIV-ATB, HIV-LTB and HIV in patients with CD4 + count <350 cells/µl. * P<0.05 and ** P<0.01. HIV, human immunodeficiency virus; LTB, latent tuberculosis; ATB, active tuberculosis; ns, not significant.

Article Snippet: PIMA™ CD4 cartridge was inserted immediately into the Alere PIMA CD4 analyzer (Alere, Inc.) and CD4 + cell count was automatically calculated.

Techniques: Virus

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: List of stabilized whole blood products tested.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: List of CD4 enumeration platforms and antibody reagents tested with commercial stabilized whole blood products.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Mean ± Standard Deviation of CD4 T-cell absolute count measurements for each stabilized whole blood product on the different enumeration platforms.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Mean ± Standard Deviation of CD4 T-cell percentage measurements for each stabilized whole blood product on the different enumeration platforms.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Ratios of mean value obtained for each stabilized whole blood product (SWBP) over mean reference value from CD4 absolute count measurements.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Low CD4 level SWBP and fresh whole blood stained with CD4FITC/CD8PE/CD3PerCP antibody combination are shown. Two dot plots are shown for each analysis: CD3×CD4 with attractor gate on CD3+4− cells cluster; CD4×CD8 (upper right corner) with attractor gate on beads, CD4, CD8 and double positive CD4+8+ cells cluster.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques: Staining

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Residual values obtained for each stabilized whole blood product (SWBP) from CD4 percentages measurements.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Stabilized whole blood products (low CD4 level) and fresh whole blood stained with CD4% easy count on CyFlow Counter are shown. Each CD4×SSC dot plot displays two gates: (1) “CD4” gate set around CD4 lymphocytes cluster and (2) “LYM” gate set around all lymphocytes.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques: Staining

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Stability of Multi-Check , StatusFlow and CD4 Count stabilized whole blood products (SWBPs) under different conditions on various platforms.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Multi-Check , StatusFlow and CD4 Count (low CD4 level) were prepared with MultiTest reagent CD3FITC/CD8PE/CD45PerCP/CD4APC on product incubated for 1 (D1) and 2 (D2) days at 37°C. Analysis displayed CD45×SSC dot plots with automated CD45 gates.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques: Incubation

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Multi-Check , StatusFlow and CD4 Count (low CD4 level) were prepared using the CD3/CD4 reagent kit on product stored at 4°C (D0) and products stored for 1 day at 37°C (D1). Analysis required first setting cursors around the CD3 cells population FSC×CD3 PECy5 dot plot and then isolating the CD4 positive cells cluster on CD4PE×CD3PECy5.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Diagnostic performance and usability of the VISITECT CD4 semi-quantitative test for advanced HIV disease screening

doi: 10.1371/journal.pone.0230453

Figure Lengend Snippet: Characteristics of study participants in Phase 1.

Article Snippet: For PIMA CD4 POC testing; the finger prick (FP) blood sample was added to the Pima CD4 reagent cartridge and the cartridge was inserted into the Abbott PIMA CD4 device ( formerly Alere ) and processed following manufacture instructions and laboratory SOPs [ ].

Techniques:

Journal: PLoS ONE

Article Title: Diagnostic performance and usability of the VISITECT CD4 semi-quantitative test for advanced HIV disease screening

doi: 10.1371/journal.pone.0230453

Figure Lengend Snippet: Contingency table of Visitect CD4 LFA test results with BD FACScount.

Article Snippet: For PIMA CD4 POC testing; the finger prick (FP) blood sample was added to the Pima CD4 reagent cartridge and the cartridge was inserted into the Abbott PIMA CD4 device ( formerly Alere ) and processed following manufacture instructions and laboratory SOPs [ ].

Techniques:

Journal: PLoS ONE

Article Title: Diagnostic performance and usability of the VISITECT CD4 semi-quantitative test for advanced HIV disease screening

doi: 10.1371/journal.pone.0230453

Figure Lengend Snippet: Diagnostic performance of Visitect CD4 LFA at different CD4 cut-offs of the reference test.

Article Snippet: For PIMA CD4 POC testing; the finger prick (FP) blood sample was added to the Pima CD4 reagent cartridge and the cartridge was inserted into the Abbott PIMA CD4 device ( formerly Alere ) and processed following manufacture instructions and laboratory SOPs [ ].

Techniques: Diagnostic Assay

Journal: PLoS ONE

Article Title: Diagnostic performance and usability of the VISITECT CD4 semi-quantitative test for advanced HIV disease screening

doi: 10.1371/journal.pone.0230453

Figure Lengend Snippet: Perception of the risk of operator-errors in the Visitect CD4 LFA testing workflow among testers.

Article Snippet: For PIMA CD4 POC testing; the finger prick (FP) blood sample was added to the Pima CD4 reagent cartridge and the cartridge was inserted into the Abbott PIMA CD4 device ( formerly Alere ) and processed following manufacture instructions and laboratory SOPs [ ].

Techniques:

Journal: AIDS Research and Human Retroviruses

Article Title: Field Performance of PIMA Point-of-Care Machine for CD4 Enumeration Under a Mobile HIV Counseling and Testing Program in Remote Fishing Communities of Lake Victoria, Uganda

doi: 10.1089/aid.2018.0223

Figure Lengend Snippet: Participant Characteristics

Article Snippet: The blood was measured using a calibrated single-channel volumetric pipette into the sample collector of the Alere Pima CD4 test cartridge and the test cartridge inserted in the PIMA CD4 Analyzer.

Techniques:

Journal: AIDS Research and Human Retroviruses

Article Title: Field Performance of PIMA Point-of-Care Machine for CD4 Enumeration Under a Mobile HIV Counseling and Testing Program in Remote Fishing Communities of Lake Victoria, Uganda

doi: 10.1089/aid.2018.0223

Figure Lengend Snippet: PIMA CD4 Count Results Versus FACSCount

Article Snippet: The blood was measured using a calibrated single-channel volumetric pipette into the sample collector of the Alere Pima CD4 test cartridge and the test cartridge inserted in the PIMA CD4 Analyzer.

Techniques: Cell Counting

Journal: AIDS Research and Human Retroviruses

Article Title: Field Performance of PIMA Point-of-Care Machine for CD4 Enumeration Under a Mobile HIV Counseling and Testing Program in Remote Fishing Communities of Lake Victoria, Uganda

doi: 10.1089/aid.2018.0223

Figure Lengend Snippet: Bland–Altman plot comparing the difference between FACSCount and PIMA versus the mean of the two methods for CD4 cell count (cells/mm3).

Article Snippet: The blood was measured using a calibrated single-channel volumetric pipette into the sample collector of the Alere Pima CD4 test cartridge and the test cartridge inserted in the PIMA CD4 Analyzer.

Techniques: Cell Counting

Journal: AIDS Research and Human Retroviruses

Article Title: Field Performance of PIMA Point-of-Care Machine for CD4 Enumeration Under a Mobile HIV Counseling and Testing Program in Remote Fishing Communities of Lake Victoria, Uganda

doi: 10.1089/aid.2018.0223

Figure Lengend Snippet: Sensitivity, Specificity, Negative Predictive Value, and Positive Predictive Value at 200, 350, and 500 Cells/μL Threshold

Article Snippet: The blood was measured using a calibrated single-channel volumetric pipette into the sample collector of the Alere Pima CD4 test cartridge and the test cartridge inserted in the PIMA CD4 Analyzer.

Techniques:

Journal: AIDS Research and Human Retroviruses

Article Title: Field Performance of PIMA Point-of-Care Machine for CD4 Enumeration Under a Mobile HIV Counseling and Testing Program in Remote Fishing Communities of Lake Victoria, Uganda

doi: 10.1089/aid.2018.0223

Figure Lengend Snippet: ROC analysis for sensitivity and specificity of PIMA using FACSCount as a gold standard, in classifying patients with CD4 T cell count of (A) <200 cells/mm3, (B) <350 cells/mm3 and (C) <500 cells/mm3. ROC, receiver operating characteristic.

Article Snippet: The blood was measured using a calibrated single-channel volumetric pipette into the sample collector of the Alere Pima CD4 test cartridge and the test cartridge inserted in the PIMA CD4 Analyzer.

Techniques: Cell Counting